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β catenin cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β catenin cell signaling technology
    β Catenin Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/active+%CE%B2+catenin+cell+signaling+technology/Non-phospho+(Active)+beta-Catenin+(Ser45)+XP+Rabbit+mAb/pm41222413-73-79-80
    Average 96 stars, based on 310 article reviews
    β catenin cell signaling technology - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Inhibition of Dickkopf-1 enhances the anti-tumor efficacy of sorafenib via inhibition of the PI3K/Akt and Wnt/β-catenin pathways in hepatocellular carcinoma
    Article Snippet: .. Primary and secondary anti-bodies used in this study Antibodies Company Product Number Dilution DKK1 R&D Systems Santa Cruz Biotechnology AF1096 SC374574 1:200 (WB) 1:50 (IHC, IF) cleaved Caspase-3 Cell Signaling Technology 9661 1:1000 (WB) 1:400 (IF) cleaved PARP Cell Signaling Technology 9544 1:1000 (WB) P110α Cell Signaling Technology 4249 1:1000 (WB) Akt Cell Signaling Technology 9272 1:1000 (WB) p-Akt Cell Signaling Technology 4060 1:2000 (WB) 1:400 (IF) β-catenin Cell Signaling Technology 8480 1:1000 (WB) Active β-catenin Cell Signaling Technology 8814 1:1000 (WB) 1:800 (IF) GSK3β Cell Signaling Technology 9315 1:1000 (WB) p-GSK3β (Ser9) Abcam ab107166 1:1000 (WB, IHC) p-GSK3β (Tyr216) Abcam Ab75745 1:1000 (WB) 1:200 (IHC) GAPDH Cell Signaling Technology 2118 1:2000 (WB) Ki-67 Cell Signaling Technology 9449 1:800 (IF) Vimentin Cell Signaling Technology 5741 1:100 (IF) N-cadherin Cell Signaling Technology 13116 1:200 (IF) E-cadherin Cell Signaling Technology 14472 1:200 (IF) Cyclin D1 Cell Signaling Technology 2978 1:1000 (WB) Goat AntiRabbit IgG Dako P0448 1:2000 (WB, IHC, IF) Rabbit AntiGoat IgG Dako P0449 1:2000 (WB) Horse AntiMouse IgG Cell Signaling Technology 7076 1:2000 (IF) Supplementary Figure 2. ..

    Article Title: KIFC3 promotes the proliferation, migration and invasion of non-small cell lung cancer through the PI3K/AKT signaling pathway
    Article Snippet: .. Email: ryzhou@cmu.edu.cn Table S1 KIFC3 siRNA sequences siRNA name siRNA sequences si-KIFC3-001 5'-CGCTGCGGAGATCTACAAT-3' si-KIFC3-002 5'-TGGAGTCGTCCAAGACCAA-3' si-KIFC3-003 5'-ACACTAGCGAGACGCTCTA-3' Table S2 Antibodies in Western blot Protein name Manufacturer Cat No. Dilution KIFC3 Abcam ab154419 1:500 GAPDH Proteintech 10494-1-AP 1:10000 PI3Kp85α Cell Signaling Technology 4257 1:1000 p-Akt Proteintech 66444-1-Ig 1:500 Akt Santa Cruz Biotechnology sc-8312 1:100 cyclin D1 Proteintech 60186-1-Ig 1:500 CDK4 Santa Cruz Biotechnology sc-260 1:500 CDK6 Proteintech 14052-1-AP 1:500 RhoA Proteintech 10749-1-AP 1:500 RhoC Proteintech 10632-1-AP 1:500 MMP2 Cell Signaling Technology 40994 1:1000 p-ERK Cell Signaling Technology 9101 1:1000 E-Cadherin Proteintech 20874-1-AP 1:500 active β-catenin Cell Signaling Technology 19807 1:1000 Table S3 PCR primer sequences gene name primer sequences KIFC3 5'-GCAGATTGCCATGTACGAGTC-3' (Forward) 5'-CGGACGCCTGCTAGATTCTC -3 ' (Reverse) PI3K 5'-GGGGATGATTTACGGCAAGATA-3' (Forward) 5'-CACCACCTCAATAAGTCCCACA-3' (Reverse) GAPDH 5'-AGGTCGGTGTGAACGGATTTG-3' (Forward) 5'-TGTAGACCATGTAGTTGAGGTCA-3' (Reverse) ..

    Immunohistochemistry:

    Article Title: Inhibition of Dickkopf-1 enhances the anti-tumor efficacy of sorafenib via inhibition of the PI3K/Akt and Wnt/β-catenin pathways in hepatocellular carcinoma
    Article Snippet: .. Primary and secondary anti-bodies used in this study Antibodies Company Product Number Dilution DKK1 R&D Systems Santa Cruz Biotechnology AF1096 SC374574 1:200 (WB) 1:50 (IHC, IF) cleaved Caspase-3 Cell Signaling Technology 9661 1:1000 (WB) 1:400 (IF) cleaved PARP Cell Signaling Technology 9544 1:1000 (WB) P110α Cell Signaling Technology 4249 1:1000 (WB) Akt Cell Signaling Technology 9272 1:1000 (WB) p-Akt Cell Signaling Technology 4060 1:2000 (WB) 1:400 (IF) β-catenin Cell Signaling Technology 8480 1:1000 (WB) Active β-catenin Cell Signaling Technology 8814 1:1000 (WB) 1:800 (IF) GSK3β Cell Signaling Technology 9315 1:1000 (WB) p-GSK3β (Ser9) Abcam ab107166 1:1000 (WB, IHC) p-GSK3β (Tyr216) Abcam Ab75745 1:1000 (WB) 1:200 (IHC) GAPDH Cell Signaling Technology 2118 1:2000 (WB) Ki-67 Cell Signaling Technology 9449 1:800 (IF) Vimentin Cell Signaling Technology 5741 1:100 (IF) N-cadherin Cell Signaling Technology 13116 1:200 (IF) E-cadherin Cell Signaling Technology 14472 1:200 (IF) Cyclin D1 Cell Signaling Technology 2978 1:1000 (WB) Goat AntiRabbit IgG Dako P0448 1:2000 (WB, IHC, IF) Rabbit AntiGoat IgG Dako P0449 1:2000 (WB) Horse AntiMouse IgG Cell Signaling Technology 7076 1:2000 (IF) Supplementary Figure 2. ..

    Polymerase Chain Reaction:

    Article Title: KIFC3 promotes the proliferation, migration and invasion of non-small cell lung cancer through the PI3K/AKT signaling pathway
    Article Snippet: .. Email: ryzhou@cmu.edu.cn Table S1 KIFC3 siRNA sequences siRNA name siRNA sequences si-KIFC3-001 5'-CGCTGCGGAGATCTACAAT-3' si-KIFC3-002 5'-TGGAGTCGTCCAAGACCAA-3' si-KIFC3-003 5'-ACACTAGCGAGACGCTCTA-3' Table S2 Antibodies in Western blot Protein name Manufacturer Cat No. Dilution KIFC3 Abcam ab154419 1:500 GAPDH Proteintech 10494-1-AP 1:10000 PI3Kp85α Cell Signaling Technology 4257 1:1000 p-Akt Proteintech 66444-1-Ig 1:500 Akt Santa Cruz Biotechnology sc-8312 1:100 cyclin D1 Proteintech 60186-1-Ig 1:500 CDK4 Santa Cruz Biotechnology sc-260 1:500 CDK6 Proteintech 14052-1-AP 1:500 RhoA Proteintech 10749-1-AP 1:500 RhoC Proteintech 10632-1-AP 1:500 MMP2 Cell Signaling Technology 40994 1:1000 p-ERK Cell Signaling Technology 9101 1:1000 E-Cadherin Proteintech 20874-1-AP 1:500 active β-catenin Cell Signaling Technology 19807 1:1000 Table S3 PCR primer sequences gene name primer sequences KIFC3 5'-GCAGATTGCCATGTACGAGTC-3' (Forward) 5'-CGGACGCCTGCTAGATTCTC -3 ' (Reverse) PI3K 5'-GGGGATGATTTACGGCAAGATA-3' (Forward) 5'-CACCACCTCAATAAGTCCCACA-3' (Reverse) GAPDH 5'-AGGTCGGTGTGAACGGATTTG-3' (Forward) 5'-TGTAGACCATGTAGTTGAGGTCA-3' (Reverse) ..



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    Assessment of kidney AKI and fibrosis in L‐G6PC1‐low and L‐G6PC1‐high mice. L‐G6PC1‐low and L‐G6PC1‐high mice were generated from G6pc −/− mice and analyzed at 12 weeks of age. Age‐matched G6pc +/+ and G6pc +/− mice with a similar phenotype served as controls. (A) Western‐blot analyzes and quantitation of renal levels of E‐cadherin and N‐cadherin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (B) Western‐blot analyzes and quantitation of renal levels of Dkk3 and CTGF in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (C) Western‐blot analyzes and quantitation of renal levels of total (β‐catenin‐T) and active, dephosphorylated (β‐catenin‐A) β‐catenin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. For Western‐blot analyzes, densitometric quantification was performed and normalized against β‐Actin. Values represent the mean ± SEM. * p < 0.05, ** p < 0.005. (D) Immunohistochemical analysis of renal levels of active β‐catenin. Scale bar, 50 μm. (E) Quantitation of renal levels of nuclear localized active β‐catenin in control ( n = 3), L‐G6PC1‐low ( n = 3), and L‐G6PC1‐high ( n = 3) mice. Kidney sections were immunostained with HRP‐labeled anti‐active β‐catenin and the nuclei counterstained with hematoxylin. Images were digitized using the Motic EasyScan Infinity 60 scanner and analyzed with QuPath software (v0.4.3). Multiple annotations were selected across the entire renal cortex. The Nucleus DAB OD mean scoring method was used to identify moderate and strong optical density thresholds for nuclear‐stained β‐catenin.

    Journal: Journal of Inherited Metabolic Disease

    Article Title: Liver‐Directed Gene Therapy Mitigates Early Nephropathy in Murine Glycogen Storage Disease Type Ia

    doi: 10.1002/jimd.70048

    Figure Lengend Snippet: Assessment of kidney AKI and fibrosis in L‐G6PC1‐low and L‐G6PC1‐high mice. L‐G6PC1‐low and L‐G6PC1‐high mice were generated from G6pc −/− mice and analyzed at 12 weeks of age. Age‐matched G6pc +/+ and G6pc +/− mice with a similar phenotype served as controls. (A) Western‐blot analyzes and quantitation of renal levels of E‐cadherin and N‐cadherin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (B) Western‐blot analyzes and quantitation of renal levels of Dkk3 and CTGF in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. (C) Western‐blot analyzes and quantitation of renal levels of total (β‐catenin‐T) and active, dephosphorylated (β‐catenin‐A) β‐catenin in control ( n = 20), L‐G6PC1‐low ( n = 23), and L‐G6PC1‐high ( n = 23) mice. For Western‐blot analyzes, densitometric quantification was performed and normalized against β‐Actin. Values represent the mean ± SEM. * p < 0.05, ** p < 0.005. (D) Immunohistochemical analysis of renal levels of active β‐catenin. Scale bar, 50 μm. (E) Quantitation of renal levels of nuclear localized active β‐catenin in control ( n = 3), L‐G6PC1‐low ( n = 3), and L‐G6PC1‐high ( n = 3) mice. Kidney sections were immunostained with HRP‐labeled anti‐active β‐catenin and the nuclei counterstained with hematoxylin. Images were digitized using the Motic EasyScan Infinity 60 scanner and analyzed with QuPath software (v0.4.3). Multiple annotations were selected across the entire renal cortex. The Nucleus DAB OD mean scoring method was used to identify moderate and strong optical density thresholds for nuclear‐stained β‐catenin.

    Article Snippet: Nuclear‐translocated active β‐catenin was analyzed by immunohistochemistry on paraffin‐embedded mouse kidney sections using a rabbit monoclonal antibody specific for non‐phosphorylated (active) β‐catenin (#8814, Cell Signaling Technology).

    Techniques: Generated, Western Blot, Quantitation Assay, Control, Immunohistochemical staining, Labeling, Software, Staining